An instrument that is contaminated with blood, body fluids, and other liquid specimens that may contain a virulent pathogen, such as Ebola virus or a biothreat agent, represents a safety hazard to personnel and the environment.
For liquid specimens, ~90 µL of each specimen was diluted with 10 vol of PBST; the cells were recovered by centrifugation (14, 000 X g for 3 min).
We have developed a microfabricated desalting device that meets both requirements, thus providing the foundational technology piece for transient ESI-MS measurements of complex biological liquid specimens.
Specimen consistency was defined according to the Bristol Stool Chart as follows: liquid specimens were defined as type 7; partially formed and soft specimens were defined as types 4 6; formed specimens were defined as types 1 3 [ 19, 22].
For the 203 male cases, three urethral discharge specimens (151 outpatients, 74.38%) or three prostate massage liquid specimens (52 outpatients, 25.62%) were collected with sterile rayon swabs (Copan, Brescia, Italy).
However, our RT-PCR assays have been shown to detect variant viruses, have been validated for sensitivity (≈100 viral genome copies per mL in liquid specimens), and provide high specificity by probe detection (5, 6 ).
The use of dry brush devices may have advantages over cervico-vaginal lavage devices, as brushes can be transported and stored dry, whereas liquid specimens are less convenient for sending by regular mail (van Baars et al, 2012).
Using these for mass spectrometry imaging experiment provide additional information in the way of spatial resolution, but may lack the sensitivity or precision of conventional protein mass spectrometry.> Whether the specimens were tissue or liquid, the specimen could be analyzed for either proteins or metabolites.
Cryo-EM images were collected in a JEM3200FSC electron cryo-microscope (JEOL, Tokyo) operated at 300 keV, and at liquid nitrogen specimen temperature.
For samples diagnosed as ASCUS, LSIL or HSIL, HPV testing can be performed on the remaining stored liquid cytology specimen without the cost of a clinical follow-up visit.
