When we find candidates, small samples are brought to the boat and immediately frozen in a liquid nitrogen dry shipper, designed to transport biological specimens at a temperature more than 150 degrees Celsius colder than the average household freezer.
After each day of collection, frozen samples were transferred to a liquid nitrogen vapour shipper for storage and transport within 6 days to a −80°C freezer at the Menzies School of Health Research laboratory in Darwin NT, Australia.
PBMC and peptides were shipped in liquid nitrogen dry shippers using IATA approved methods.
Mosquitoes were killed on dry ice and placed in liquid nitrogen dry shippers or on dry ice in insulated containers for transport to Cairns for storage at –70oC.
Half of the samples were placed in Nunc CryoTube vials (Nalge International, Rochester, New York, USA), which were placed in a small liquid nitrogen dry-shipper container (5.4 L) for cryopreservation (–196°C).
Originally, the frozen samples were transported by air and land in dry shippers in liquid nitrogen vapor from Townsville, Australia to the CryoDiversity Bank at Taronga's Western Plains Zoo in Dubbo, a distance of 1775 km.
The two most popular uncontrolled cooling devices were used in this study: a Styrofoam box setup with a polystyrene floating raft on liquid nitrogen and the dry shipper setup with a straw holder.
Tubes were placed in a dry shipper filled with liquid nitrogen, transported to the lab and processed within 24 h after sampling.
Approximately 25 50 × 10 mononuclear cells (MNCs) were collected from each of 25 donors into 10 bags by apheresis, cryopreserved in 10% dimethyl sulfoxide (DMSO), then shipped to the UK in vapour phase liquid nitrogen by an accredited shipper with temperature logging throughout.
Here we report on the efficacy of microbial decontamination of the liquid nitrogen (LN) dry (vapour) shippers used for short-term storage and transportation of germplasm and other biological specimens.
Shipping of the samples from European sites is carried out using styrofoam boxes filled with dry ice and from Ghana in dry shippers filled with liquid nitrogen.
