149Tb-DOTANOC was obtained with >98 % radiochemical purity at a high specific activity (5 MBq/nmol), as confirmed by high performance liquid chromatography (HPLC -based quality control.
OCT: Octenidine dihydrochloride; PH: 2-Phenoxyethanol; MRSA: Methicillin-resistant Staphylococcus aureus; LOD: Limit of detection; LOQ: Limit of quantification; HPLC: High-performance liquid-chromatography; HQS: High quality control standards; LQS: Low quality control standards.
Moreover, the analysis using LCMS, GC/MSD, and LC-MS is very expensive and delicate as compared to ultra-performance liquid chromatography (UPLC) for routine quality control analysis.
High performance liquid chromatography (HPLC) purification and quality control were performed on a semi-preparative column (C18, 5 μm, 250 × 10 mm), or an analytical column (C18, 5 μm, 250 × 4.6 mm) respectively, both purchased from Phenomenex (Torrance, CA) and used on an Agilent 1260 infinity platform (Santa Clara, CA).
Two levels of quality control material (Liquid Assayed Multiqual®, Bio-Rad Laboratories, Irvine, CA, USA, Cat. No. 694, 695) were analysed daily prior to running the samples.
Our data show that the system can achieve time savings measured in days for key quality control applications (filterable liquid, air, and surface samples).
Headspace extraction used for volatile analytes in solid or liquid samples is preferable technique for a quality control but the sensitivity is limited (Pawliszyn 2000).
For example, flow cytometric methods [5] only operate on small liquid samples (generally less than about 0.5 ml), and cannot test air, surface, or large volume liquid samples which are critical in manufacturing quality control.
To improve its quality control approach, an ultra-performance liquid chromatography (UPLC) fingerprint method was developed for rapidly evaluating the quality of Resina Draconis.
As a result, the employment of qualitative or quantitative chromatographic techniques such as high-performance liquid chromatography (HPLC) could be very useful for quality control of TCHM.
