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The other two parts, the water block and liquid plate, are universal and should work in nearly any system.
Plates were then washed and incubated with 200 µL of a sealer/stabilizer solution 'Liquid Plate Sealer' (Candor Bioscience) for 1 h at 37 °C and then aspirated.
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AH109 cells (leu2- trp1- ade2- his3-) were grown in YEPDA liquid plates or on YEPDA plates with 17 g/L Agar-Y (Bio101 Systems).
rad52∆ estrainsrains entered senescence and failed to recover, as expected, but interestingly showed different growth patterns in solid vs. liquid plates.
Numerical calculations were carried out for various kinds of system parameters: i.e., the radius to thickness ratio, the liquid-plate density ratio, plate material parameters, and the stiffness of the elastic foundation.
We then used liquid pressure, and the out-of-plane deflection of the thin plate for the construction of frequency response functions for the analysis of vibration characteristics in the liquid-plate coupling system.
A laboratory-scale workstation with basic liquid and plate handling capacity would be able to dispense hESC suspensions into microtiter plates, incubate them, and recover the aggregates with no operator intervention.
Carefully pour liquid from plate into bowl.
The active components are cooled with a liquid cold plate.
The designed liquid cold plate (LCP) contains these simple oblique fins with optimized angle and width.
Both liquid and plate cultures were grown under microaerophilic conditions (5% O2, 10% CO2, and 85% N2) generated with an Anoxomat gas evacuation and replacement system (Spiral Biotech) in gas evacuation jars.
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