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For metabolomic profiling, 60 mg of frozen plant tissue was extracted with methanol/chloroform, and the polar fraction was prepared by liquid partitioning into water and derivatized [46].
Fig. 1A depicts 3 g of A2E prepared using liquid partitioning.
Fig. 1C shows a representative UV-VIS trace of A2E purified by liquid partitioning.
The preparation employs a one-step biomimetic preparation from all- trans-retinal and ethanolamine, first described by Parish et al. (Parish et al., 1998), but exploits liquid partitioning to isolate A2E.
In recent years, extraction methods have usually been based on liquid partitioning with ultrasonic extraction (USE), microwave-assisted extraction (MAE) or with the more advanced techniques of pressurised liquid extraction (PLE), or supercritical fluid extraction (SFE), which have replaced Soxhlet extraction [ 57, 58].
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The crude extract was suspended in water and fractionated by liquid: liquid partition with solvents of increasing polarity; starting from n-hexane, ethyl acetate and chloroform.
The crude extract was suspended in water and fractionated by liquid: liquid partition with solvents of increasing polarity; starting from n-hexane (23 g; LPHE), ethyl acetate (43 g; LPEE) and chloroform (67 g; LPCE).
Extraction, liquid partition, and derivation prior to GC-MS analysis were performed as described by Lisec et al. [ 35].
Liquid-liquid partitioning methods will become more and more conmntinous operations.
Liquid-liquid partitioning methods including solvent extraction and aqueous two-phase partitioning are very useful tools for bioseparations.This chapter provided the methodology with various examples and principles as guidelines for the liquid-liquid partitioning in a practical application.
The procedure involves a single-phase extraction of the analyte with acetonitrile, following liquid-liquid partitioning, accomplished by the addition of anhydrous MgSO4 and NaCl.
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