A1C was measured in the central core laboratory on an automated high-performance liquid chromatography analyzer (Bio-Rad Variant Analyzer; GMI, Ramsey, MN) using a Diabetes Control and Complications Trial aligned assay.
Timed reactions were stopped with an equal volume of 2× STOP solution (40 mM EDTA and 1% SDS) and spotted on DE81 filter papers, then analyzed using a Perkin Elmer Liquid Scintillation Analyzer (Perkin Elmer, Tri-Carb 2910 TR).
Dried filters were each placed in a vial with 5 mL scintillation fluid (Scintisafe; Fisher, Fair Lawn, NJ) and were analyzed by a Tri-Carb 2100TR Liquid Scintillation Analyzer (Packard Instruments, Downers Grove, IL).
We placed dried filters each in a vial with 5 mL scintillation fluid (Scintisafe; Fisher) and analyzed them by a Tri-Carb 2100TR Liquid Scintillation Analyzer (PerkinElmer).
The radioactivity in baseline and stimulation fractions as well as radioactivity in the brain slices at the end of the experiment was analyzed using a Packard 1900 CA TRI-CARB liquid scintillation analyzer.
LSC measurements were performed using a Packard Tri Carb liquid scintillation analyzer or a Hidex 300 SL.
The 3H concentrations in groundwater were measured after electrolytic enrichment, using a liquid scintillation analyzer (Packard 3100TR, PerkinElmer 1220 Quantulus (PerkinElmer Inc., Waltham, MA, USA), or LSC-LB5 (Hitachi Aloka Medical, Ltd., Mitaka, Tokyo, Japan).
Total radioactivity was measured in a TriCarb 2800 TR Liquid Scintillation analyzer (Perkin Elmer).
Cells were then lysed with 1% sodium dodecyl sulfate and the radioactive incorporation was determined with a liquid scintillation analyzer.
Four ml Ecoscint scintillation cocktail was added to each lysates and [3H] was counted in a Liquid Scintillation Analyzer.
The cell-associated radioactivity was counted with a liquid scintrillation analyzer (Tri-Carb 2800 TR, Perkin Elmer).
