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Oil Red O staining was also performed to visualize lipid formation.
Moreover, adipocyte size and lipid formation in the liver were examined.
Upon correction for possible impedance signal interferences, we derived different empirical methods suitable to indicate incipient lipid formation.
A kinetic model of lipid formation under steady state conditions was developed, parameters estimated, and optimal continuous cultivation conditions forecasted.
We found that weight gain, epididymal fat pad weight, adipocyte size, and lipid formation were markedly attenuated in the livers of HFD-induced obese mice treated with ECE.
Under nitrogen limitation, the highest lipid yield of 0.19 g/g was observed at the dilution rate of 0.02 h−1 while the highest specific lipid formation rate of 0.058 g/g cell/h at the dilution rate of 0.08 h−1.
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Clam tissues were also examined for the presence of oxidative damage to lipids, formation of DNA strand breaks, and alterations in heme metabolism.
These findings indicated that miR-210 promotes the lipids formation by repressing WNT signaling through targeting Tcf7l2.
Lipid droplet formation was confirmed by oil red O staining.
Relative quantification of lipid droplet formation was determined by absorbance measurement at 500 nm.
Manickam, E., Sinclair, A. J. & Cameron-Smith, D. Suppressive actions of eicosapentaenoic acid on lipid droplet formation in 3T3-L1 adipocytes.
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