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The fluorescence intensity corresponding to the intracellular lipid amount was determined at the peak of the corrected spectrum.
The first strain SSOH12 was identified as Pichia segobiensis producing a considerable amount of 16% palmitoleic acid (C16:1) of total lipid amount.
Therefore, in their investigation related to the effect of medium pH on lipid production by Rhodosporidium toruloides strain, (Dias et al. 2016) found that yeast biomass concentration and lipid amount were the highest at pH 4.0.
For lipid hydrocolloid emulsion based film, the increase of lipid amount would improve its water vapor barrier property, but also reduce the mechanical strength of the film in the meantime thus leading to a compromised lipid content in the film.
The Box Behnken experimental design is used to analyze the influence of three factors (X1 = bovine serum albumin concentration, X2 = pH of the aqueous phase, and X3 = lipid amount) on the particle mean diameter (PMD) measured by dynamic light scattering (DLS).
Samples Lipid Amount Oxidation rate* (10-8M s-1m-2) % Oxidation Suppression# A. ferrooxidans/Pyrite N/A 1.62 ± 0.1 N/A A. ferrooxidans/Lipid/Pyrite 7.5 μmol 0.30 ± 0.02 81 Lipid/Pyrite 7.5 μmol 0.088 ± 0.005 95 Pyrite N/A 0.21 ± 0.01 N/A N/A denotes Not Applicable *Rates are estimated from a linear regression analysis of the final 10 days of data in Figure 1.
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The relative intensities against the unit lipid amounts were almost similar except for one yeast.
Due to sufficient production of lipid amounts and interesting fatty acid profiles, further studies of all three strains are worthwhile to establish sustainable bioprocesses for the production of adequate amounts of microbial oil for industrial applications.
Quantification of the lipid amounts was performed by scanning the plates with a Hewlett Packard Scanjet 4500c and by integrating the density areas with Tina version 2.09 software.
Lipid amounts (pmol) were corrected for response differences between internal standards and endogenous lipids.
Exposure of the cells to different lipid amounts always generated the same fluorescent protein pattern.
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