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To stop the cross linking reaction, glycine (Sigma-Aldrich, G-7403) was added to a final concentration of 0.125 M.
The majority of the crosslinked species was the slowest migrating species (oligomer 2) suggesting that the oligomer 1 form may be an intermediate product of cross linking reaction.
The DNA/protein cross linking reaction was stopped by adding a glycine stop-fix solution.
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Heat accelerated the cross-linking reaction, probably by elevating the cross-linked solution flux into the matrix.
It was observed that increasing the concentration of glutaraldehyde in the cross-linking reaction resulted in an increase in the cross-linked HP0593 MTase.
For each complex, the cross-linking reaction was repeated twice on different samples, which afforded almost identical cross-links.
The hydrogel polymeric network is formed via a laccase-mediated cross-linking reaction.
To terminate cross-linking reaction, cells were incubated with 0.125 M glycine for 5 min while rotating.
Residues in red indicate the site of primary amines involved in the cross-linking reaction using either DSS or BS3.
A cross-linking reaction yield of up to 50% was obtained.
The cross-linking reaction was quenched with a final concentration of 50 mM ammonium bicarbonate at 37 °C, 500 rpm, 30 min.
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