BMP7 binds ActRII with 3-fold higher affinity compared to BMP2 (Kd = 1.7 μM versus 5.4 μM) and binds ActRI/ALK2 only with low affinity (Kd = 143 μM)57.
IgG antibodies recognize antigens with high affinity and bind cellular Fc receptors with low affinity individually.
BIAcore analysis demonstrated that, with the extracellular domain (ECD) of NP-1 immobilized at low density, VEGF165 bound with low affinity (K d) = 2 microm) and fast kinetics.
It has been reported that ovarian cancer G protein-coupled receptor 1 (OGR1) is a high affinity receptor for SPC, and its closely related homologue GPR4 is a high affinity receptor for SPC with low affinity for lysophosphatidylcholine (LPC).
The reconstituted SPR bound the SPR agonist substance P, 125I-labeled with Bolton-Hunter reagent, with low affinity.
It also utilizes dUMP as substrate however with low affinity than dTMP [44].
Biochemical evidence indicated that Noggin binds to BMP2 with high affinity but to BMP7 with low affinity.
Aplindore shows high affinity for D2 and D3Rs, with low affinity for serotonin receptors and adrenoreceptors.
Importantly, ADP binds with low affinity, which is insufficient to interfere with binding or release of these high affinity inhibitors.
This difference in electron affinity may allow PDIs to selectively break disulfides with high electron affinity (incorrect bonds), but not disulfide bonds with low affinity (correct bonds).
