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The effect of WOX1 on responsive promoters driven by transcription factors was determined using the following reporter constructs (Stratagene), as described [31], [32]: (a) Luciferase reporter gene linked to tandem repeats (4X) of CRE and AP-1, respectively; (b) A Gal4 reporter system specific for transactivational activity of CREB, c-Jun and c-Elk-1, respectively.
Unequal crossing over and gene conversion are often linked to tandem duplication, which results in arrays of similar regions of DNA.
Sugar-phosphates, nucleotide sugars and organic acids were extracted in chloroform/methanol (3:7 v/v) and measured by anion-exchange chromatography linked to tandem mass spectrometry [ 71].
In the present work, we describe a new variant of the previous construct that is based on the TDP-43 molecule itself (or fragments of it) linked to tandem repeats of the 339 369 sequences (TDP-12xQ/N).
One can conclude that a relatively high level of methylation (usually higher than genome average) is not linked to tandem arrangement but also occurs when 5 S genes are organized as single or low copy insertions.
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HEK293 cells were transfected with two separate reporter constructs containing the luciferase gene under the control of either a basally active β-actin promoter linked to six tandem Hes1-binding sites (N-box) (Fig. 4A) or a 3.0 kb fragment of the neurogenin3 promoter containing multiple N-box sequences (Fig. 4B).
The comprehensive analysis reported here has revealed multiple U3 snoRNA genes in the protist Euglena gracilis, in three distinct genomic arrangements: i) stand-alone, ii) linked to two tandem, identical tRNAArg genes, and iii) linked to a U5 snRNA gene.
It was previously demonstrated [ 13] that immunization of mice with E. coli-produced flagellin linked to four tandem copies of human "consensus" M2e, provides protection against lethal challenge with human influenza A strain.
Profilaggrin is an insoluble phosphoprotein that consists of an amino terminal Ca2+-binding protein of the S-100 family, linked to 10 12 tandem filaggrin monomer repeats (Dale et al, 1985; Harding & Scott, 1983; McGrath & Uitto, 2008).
This study utilised Gamitrinib G4 (G–G4), which contains the Hsp90 ATPase inhibitory structure of 17-AAG (LC-Laboratories, Woburn, MA, USA) linked to four tandem repeats of guanidinium, used as a mitochondriotropic moiety (Kang et al, 2009).
These rules will be applied using transaction data gleaned through third-party bank accounts you've linked to in the Tandem app (and possibly your Tandem credit card, if you have one) and include rounding up payments to the nearest pound.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com