An H3 peptide (residues 23 34) containing K27photoMet and a biotin tag cross-linked efficiently to EZH2, the catalytic subunit of PRC2, suggesting that histone mutants act as orthosteric active site-directed inhibitors.
As a positive control, we use ds-F that cross-links efficiently to M.SssI, as shown by the appearance of two bands with retarded mobility on the SDS-PAGE gel (Fig. 2B, lanes 8 and 9).
But eventually and all players are hoping for sooner rather than later, of course the firms seek to create integrated systems of PCs linked efficiently with entertainment equipment.
First, two internal deletions (Fig. 1d) that lacked sequences from this interval failed to cross-link efficiently (Additional file 2: Figure S2A).
A surface modelling technique was adopted and efficiently linked to the finite element (FE) code to simplify future FE analyses.
We can infer from this analysis that the ether group of PEG is efficiently linked to the positively charged surface of SPIONs, and mediated through a dipole-cation covalent interaction.
Information about the experiences in this specific shelter will help program and policy makers to design or adjust shelters services that adequately fit the needs of homeless populations, and are efficiently linked to the healthcare system.
However, position 184 in the second half of the CCR5 EC2, which cross-linked efficiently with 2D7, does not have any known proximal hot spots of interaction.
These were almost always observed in CRAC experiments (Granneman et al, 2009), but generally represented a larger fraction of the sequences recovered with proteins that cross-linked less efficiently to RNA.
In this way, reporter or other genes may be efficiently linked to both the 5' and 3' regulatory regions.
