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Figure 3C is a diagnostic restriction digest of the individual BACLink subclones, Trap-GM and Dmp-1/Ibsp-SP compared to the linked DNA construct (OOO-1), where the restriction digest confirms that the linked construct retains all the appropriate size DNA fragments that collectively are present in the two separate genomic subclones.
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But they are inextricably linked, constructed almost at the same time by two business partners, Frederick Edey and Henry B. Hollins, who was sometimes known as Harry.
This monitoring was conducted each month over 2009 (with the exception of March and April due to equipment failure) at the Chancellor Park estate in Sippy Downs, South East Queensland, Australia, which contains a series of ten linked, constructed urban lakes.
Recurring use of bookmarks and links may also bring attention to the potential benefits of linking within EHRs using the openEHR and ISO 13606 linking constructs in order to create for example medical history descriptions or discharge summaries without repeating too many details.
Reaction mixtures containing the native isopeptide-linked construct Isopep.SUMO-2×4, show that Ube2W attaches up to four copies of ubiquitin to this form.
The presence of substances such as hemicelluloses, lignin, and pectin and their spatial inter-links construct physical barriers to protect cellulose from degradation.
The linked BAC construct was purified from 250 mls of bacteria culture using a large construct kit (Qiagen).
The nadB::Tn10 rpoE::kan linked marker construct used in cotransduction assays was made in a strain that contains an unidentified suppressor of ΔrpoE (sup, CAG43113).
Subsequently, the inference nodes can be generated step-by-step and appropriately linked to construct hierarchical multistage inference nets.
Earlier structural studies with Mini-B, a disulfide-linked construct based on the N- and C-terminal regions of SP-B (i.e., ∼residues 8 25 and 63 78), confirmed that these neighboring domains are helical; moreover, Mini-B retains critical in vitro and in vivo surfactant functions of the native protein.
Re-analysis of the MS data for slices 1 6 of Figure 2 did indeed reveal a peptide consistent with ubiquitylation at the 6His-SUMO-2 N-terminus in the peptide-linked construct.
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