The sample was washed and the bead-binding complexes were eluted with elution buffer [ 48].
The complexes were eluted by adding elution buffer (1% SDS, 0.1 M NaHCO3).
DNA/protein complexes were eluted and decross-linked by heating at 65°C overnight [ 38, 39].
Protein-DNA complexes were eluted; cross-links were reversed by adding NaCl (200 mM) and 10 µg of RNase A and incubating at 65°C overnight.
Protein-DNA complexes were eluted in 200 µl of elution buffer and the cross-links reversed by overnight incubation at 65°C.
The immune complexes were eluted by incubation in elution buffer, and supernatants were isolated and further incubated for 4 hrs at 65°C to reverse cross-linking.
Then complexes were eluted with freshly prepared elution buffer (1% SDS, 100 mM NaHCO3).
Complexes were eluted with 500 μl elution buffer (1.0% SDS, 0.1 M NaHCO3).
After extensive washing, complexes were eluted with 500 μl elution buffer (0.1 M NaHCO3, 1% SDS).
After six washing steps, complexes were eluted and the cross-links reversed.
The antibody/protein/DNA complexes were eluted with 150 ul IP elution buffer (50 mM NaHCO3, 1% SDS) twice.
