Sentence examples similar to linked cells were washed from inspiring English sources

Cross-linked cells were washed with cold PBS and lysed by incubation for 10 min in ice-cold lysis buffer (10 mM Tris (pH 8.0), 10 mM NaCl, 0.2% NP-40, protease inhibitor cocktail R1321 (Fermentas, Vilnius, Lithuania)).

The formaldehyde crosslinker was quenched on ice by the addition of 375 mM glycine for 5 min. The cross-linked cells were washed twice in phosphate-buffered saline, harvested, and suspended in cell lysis buffer (25 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS) supplemented with protease inhibitors.

Cross linked cells were scraped, washed with PBS.

Cells were washed with PBS and UV cross-linking (800 J/cm) was carried out using a Stratalinker (Stratagene).

Briefly, cells were washed twice with PBS then labeled with EZ-Link Sulfo-NHS-SS-Biotin.

Cross-linking was stopped by the addition of glycine to a final concentration of 125 mM, and cells were washed twice with PBS.

The cells were washed with PBS, fixed, and washed again.

The harvested cells were washed and lyophilized.

And cells were washed to remove media.

Cells were washed three times with PBS (5 min for each wash).

Cells were washed three times with PBS before AFM measurements.