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The huge decrease observed in the photoluminescence (PL) quantum yield of the resulting particles indicates the formation of core-shell CdSe/TiO2 nanoparticles, which was reported as due to a photoinduced electron transfer from CdSe to TiO2 in a linked arrangement [3].
Thus, linked arrangement of rDNA arose independently in the largest subfamily.
Our results indicate that nearly 25% of Asteraceae species may have evolved unusual linked arrangement of rRNA genes.
These data suggest that a putative Elachanthemum progenitor did have linked arrangement of units that have become eventually lost.
In species displaying linked arrangement the homogenisation of linked rRNA genes most likely went to completion, since no unlinked genes were detected by FISH or Southern blot hybridisation.
In the case of a linked arrangement (5 S copies flanked by non-5 S DNA) only products corresponding to ~120-bp monomers would be amplified.
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In short, the species with homogenised linked arrangements bear apparently functional 5S genes in the 26S-18S intergenic spacer(s).
These linked arrangements are found among diverse biological taxa including nematodes [ 2], fungi [ 3], crustaceans [ 4], slime moulds [ 5] or mosses [ 6, 7], and they are believed to represent transition states between linked (prokaryotic) and unlinked (eukaryotic) arrangements.
However, our recent observations in a group of angiosperms (genus Artemisia, Asteraceae) [ 8] clearly point to the possibility that linked arrangements might not be restricted to prokaryotes and primitive eukaryotes but may occur throughout the tree of life.
To reveal chromosomal positions of rDNA loci and the degree of repeat homogenisation we conducted FISH assays in selected representatives of the Asteroideae (group where most linked arrangements have been found) and in one Cichorioideae.
We have also confirmed a linked rDNA arrangement for all the studied Tanacetum species.
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