Co-morbidity was objectively assessed by a record-linkage with previous hospitalizations, in order to assess the occurrence of previous illnesses that could be associated with the likelihood of being identified as frails or at risk.
Notably, this finding is replicated among MSMW for both male and female primary partners providing many linkages with previous qualitative and quantitative work that has shown a strong preference for disclosure to primary partners [ 8, 10, 11].
The linkage region overlaps with previous QTLs identified in [8] and [7].
For determining homologies between linkage groups and deriving consistent nomenclature of subgenomes with previous linkage analysis (Okada et al. 2010), the populations were genotyped with subsets of previously mapped markers.
Our GWA scans also found several novel QTLs that do not overlap with previous linkage analysis regions (Figure S6).
As with previous linkage assessments in observational studies [35], our protocol (Figure 1 and Table 4) included an evaluation of sequence data from env and gag for monophyly in maximum likelihood trees to determine linkage.
In contrast, but in agreement with previous linkage analysis [17], three (134, 133, and 124) out of the nine at-risk unaffected individuals (Figure 2) carried the HLA DR15/DQ6 allele but did not carry the A allele of rs4762896, while three (137, 138, and 134) with the chromosome 12 haplotype, also had the A variant but not HLA DR15/DQ6.
Correspondences with previous linkage map [ 8, 9] and with specific chromosomes based on FISH results [ 23] are given (see Additional file 1).
A04 was the smallest LG that spanned 60.6 cM and comprised of 195 loci, and C03 was the largest LG that spanned 175.0 cM with 1,138 loci, which were consistent with previous linkage maps constructed with SSR markers [ 30].
On-going genetic studies in the cohort remain to discover what genomic regions are linked to preeclampsia in the Norwegian families, and if these comply with previous linkage studies in Icelandic, Australian/New Zealand, Finnish and Dutch families.
A major leaf shape QTL (qLS1, 62% phenotypic contribution) was mapped to an 8.8-cM interval in linkage group C15 Dt)_LG04, consistent with previous results achieved in other populations (Jiang et al. 2000; Lacape et al. 2013; Andres et al. 2014).
