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All sequences revealed the typical -GCCG- or -GGCG- linkage sequence between the npt-II-T35S and the rolC fragments, thus clearly indicating ATDs excision (data not shown).
Specificity is maintained when longer Ub chains are used as substrates but depended on an intact Ub Gly-Met linkage sequence between Ub moieties, given that mutant tetraUb with Ser-Met linkages was not hydrolyzed.
The H-bonded chains are arranged into a 2D extended sheet parallel to (100) in such a way that a linkage sequence X′ X T X′ X T is generated.
Acidic amino acids surrounding these Ser-Gly repeats promote substitution with HS as well, possibly by helping the first N-acetylglucosamine (GlcNAc) transferase to act on the linkage sequence [ 23, 24].
pRetroQ-Flag-AR-T2A-mCherry, a mammalian dual non-covalently-linked AR and mCherry expression construct, was cloned by PCR addition of an N-terminal Flag-tag and C-terminal T2A linkage sequence to mouse AR cDNA using template pENTR233.1-AR pENTR233.1-AR016477, SourCloneoscIDnce) with product subcloned into pRetroQ-mCherry.
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SNP chromosomal assignment by turkey linkage, turkey sequence and chicken sequence maps NA = Not Aligned Recombination rate of turkey chromosomes varied from 1.6 to 17.2 cM/Mb.
Sanger sequencing provides linkage on sequenced isolates but only provides approximate frequencies on sequenced populations.
Negotiating the precise linkage and sequence of these elements is exactly what the two countries need to talk about.
SBT provides high-resolution typing, but at high cost and low throughput, and is not able to discern cis/trans linkage of sequence motifs, which can result in ambiguities in allele calls [43].
Data on human infection with avian influenza reported to the WHO should be more accurate, includes linkage to sequence data, and be made more readily available for investigators; countries in which cases occur need to be more proactive at investigating cases properly [39].
Linkage and sequence maps were drawn using the MapChart program [ 43].
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