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The linkage relationship between markers and the NUDA/GL-1 locus was analyzed.
The yellow-green leaf mutant DNA pool and wild-type phenotype DNA pool were used for linkage relationship analysis.
The putatively linked markers were then used for genotyping of the individual F2 plants to study linkage relationship.
These are invaluable resources in analyzing genetic diversity in a population and in establishing the linkage relationship between genomes and heritable traits (Chen et al. 2011; Osman et al. 2003).
To understand the linkage relationship between Pi2-2 and the R genes in the same region, we developed an F2 population from a cross between Jefferson and Pi9- carrying line 75-1-127 for allelism test.
To further define the linkage relationship between qSPP5 and qTGW5, we self-crossed 18 BC5F3 plants that were selected from B1, B2, B6, and B8 to produce 18 BC5F4 lines and evaluated them in terms of the TGW, SPP and CL (Figure 4B).
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The importance of these mutations in evolution is that they change the linkage relationships between genes.
Linkage relationships were analyzed by Quantitative Trait Loci (QTL /Simple Interval Mapping and Marker regression analysis with the MAPManager program (Meer et al. 2002) using the Kosambi mapping function (Kosambi 1944).
The region of falsely anchored scaffolds disturbed the normal linkage relationships in the graphic map of the genotypes and was not supported by normal syntenic analysis with the reference.
Realization of these opportunities depends on linkage relationships with the centromere (Table 4).
Measures of linkage relationships between the six loci and the centromere were obtained to quantify the relationship between relative sequence diversity and chromosomal position.
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