Sentence examples for linkage preferences from inspiring English sources

Using NMR we modeled the structure of diUb/Josephin complexes and showed that linkage preferences are imposed by the topology of the two binding sites.

To clarify linkage preferences of the Josephin domain in the absence of the C-terminal region of ataxin-3, we incubated isolated Josephin with equal amounts of K48-linked or K63-linked pentaUb chains (Fig. 3D).

It has been shown experimentally that the two isolated, contiguous ataxin-3 UIMs, which are connected by a two residue linker, contribute to binding of diUb with binding constants around 100 µM and no obvious linkage preferences [16].

The high level of conservation between the Ubc domains of UbcM2 and UbcH5 but dramatically different Ub conjugating profiles offered the opportunity to identify residues within the Ubc domain that govern the intrinsic Ub transferring properties and linkage preferences of UbcM2.

Enzymes were used at different concentrations in order to identify the lowest DUB concentration that resulted in significant cleavage of the preferred chain type(s), indicating linkage preference of the DUB.

The four amino acid substitutions in HA identified by (2) (the Imai et al. set) also contain two receptor-binding amino acid substitutions, N220K and Q222L, one of which is in common with the Herfst et al. set and which together are known to change the sialic acid linkage preference to the more human-like alpha-2-6 linkage (2).

Finally, we performed a run in which the linkage preference was left ambiguous by defining a linkage restraint including both K48 and K63.

Together, these results confirm the crucial importance of site 1 in cleaving all Ub chains and a role of site 2 in conferring linkage preference to ataxin-3.

Whether or not the current H5N1 viruses are capable of progressive mutations that switch their SA linkage preference is central to an accurate risk assessment of the potential of H5N1 to be the progenitor of a human influenza virus pandemic.

It was unclear whether OTU orthologs have conserved their linkage preference throughout evolution.

The limited polyUb chain building capacity of UbcM2 prompted us to investigate the Ub Ub linkage preference of the enzyme.