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To this end, selected SNPs in the 27 individual segregants were scored by allele-specific PCR to determine the SNP variant frequency and the statistical significance of the genetic linkage precisely.
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Dataset linkages are precisely the kind of diversity we aim to find when fusion is operated.
More precisely, linkage equilibrium means that the population-wide frequency of the AiBi gamete is equal to the frequency of the Ai allele multiplied by the frequency of the Bi allele.
Six primers able to amplify the contig linkage regions (described precisely in the Methods) unique in the two CMS lines in comparison with Nipponbare were used to detect each amplicon by PCR.
It is difficult for designers to synthesize the best linkage through rapidly and precisely selecting curve points that ensure to satisfy every design condition.
Omitting homologues to unassigned A. mellifera sequences, 10 linkage groups could be precisely matched with 4 to 26 (mean 13.7) homologous loci.
We estimated the contemporary effective population size of all populations using the linkage disequilibrium (or more precisely gametic phase disequilibrium) method [ 43, 56].
For the EXPEN 2000 linkage map to correspond precisely to cultivated tomato chromosomes, the genomes of the two species have to be structurally identical, i.e., no major inversion, translocation, duplication, or deletion differences.
Moreover, several studies have demonstrated that a map of very closely spaced SNP markers could offer many advantages over the low density maps of microsatellite markers, mainly by increasing the power to detect linkage and consequently more precisely identify the disease locus [ 2- 7].
We show that oil modifies the local environment of aluminum in this material and, more precisely, the Al OH linkage.
Therefore, the exact position of the QTL determined by linkage analysis could not be precisely determined.
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