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The binary complex structure and results of engineered disulfide linkage experiments reveal that the plug and motif B loop, which block the access of template DNA to the active site in the apo-form mini-vRNAP, undergo a large-scale conformational change upon promoter binding, explaining the restricted promoter specificity that is critical for N4 phage early transcription.
For epitope and antibody linkage experiments, six-week old female B6 mice received single immunization with selected peptides in the context of incomplete Freud's adjuvant (IFA) and CpG oligonucleotide.
Including information from all traits in a multiple-trait analysis has been reported to increase the power to detect QTLs in linkage experiments (Korol et al., 2001, Gilbert and Leroy, 2007) and association analysis (Ferreira and Purcell, 2009, Bolormaa et al., 2010).
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Linkage mapping experiments were conducted with three independent F2 populations derived from B73 and one of three other inbred lines from the maize association population (CML247, CML333, NC350).
Two melon populations were employed to conduct linkage mapping experiments using cucumber microsatellite markers.
NGS technologies have been implemented in linkage mapping experiments to develop novel and promising approaches, such as RAD-Seq, GBS, GS, and QTL-Seq.
This result highlights the promise of next-generation sequencing based genotyping, and especially the ddRADSeq protocol, for linkage mapping experiments when biological material is sufficient.
Bioinformatic analyses were performed by DS with input from RJ. RJ performed the genetic crosses, genome sequencing and linkage mapping experiments and AF contributed to the fine scale mapping.
Each of the bi- and multi-parental linkage mapping experiments detected a major and recurrent locus that had previously been mapped by Sicard et al. [ 6] and named rpv1.
In this case, QTL identified via traditional linkage mapping experiments performed on the mapping population can be immediately tested by selecting NIL s) representing the QTL introgression and testing them for a phenotypic effect relative to the wild type recurrent parent.
It is also worth noting that 23% of the previously published SNPs were not verified in our sample, despite the fact that these SNPs have previously been verified through genotyping and linkage mapping experiments (e.g.[ 3, 34]).
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