However, only a small heterozygous linkage drag was detected at the xa5 locus.
However, there is no difference in terms of linkage drag in pseudo-backcrossing schemes.
Moreover, linkage drag often occurs during MAS (Lewis et al. 2007).
In order to break the linkage drag, we tried to continuously backcross with NJ3.
The break of linkage drag would depend on a larger population in the future study.
Introduction of new traits in the bisporus variety is thus problematic due to large linkage drag.
That would allow a more precise mapping and reduction of linkage drag.
However, the use of landraces for breeding is often hampered by linkage drag.
In pseudo-backcrossing BILs, there was only a 1% reduction in linkage drag from BC1 to pseudo-BC3, and most of this reduction was due to the heterozygous segments of the linkage drag.
One of the main reasons is the extraordinary recombination landscape in the variety bisporus causing substantial linkage drag.
Our results also revealed that the degree of linkage drag is independent of the size of the target genes/QTLs.
