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Fig. 4 Linkage confirmation of qCTS3-9 in the rice collection using a newly developed indel marker.
However, it should be noted that viral sequence-based linkage confirmation performed in our study cohort was not performed in the Rakai study.
Linkage confirmation was performed in a prior study of Zambian HIV-1 serodiscordant couples [1], but the specific measures of the relationship of plasma HIV-1 and transmission risk discussed here have not been reported for that cohort.
We thank M. Fink and W. Giles for coding the automated extraction of heart beat data from traces, J. Ayroles and E. Kennerly for the SFP analysis, and P. Hunt for linkage confirmation by sequencing.
The final selected candidate markers then went through the genetic linkage confirmation step and validation step in the same way as in other standard marker development methods [ 16, 21, 37].
The linkage confirmation and validation steps ensured that the final markers recommended for MAS were single copy in the genome, were closely linked to gene of interest, were applicable to wide range of breeding germplasm, and were desirable for marker-assisted plant breeding.
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In addition, ongoing quality control measures are needed within those cohorts that rely on tumor registry linkages for confirmation of incident cancers as performance of linkages can vary over time.
Therefore data from 328 DNAs were used in the transmission and linkage analyses after confirmation.
The goal: Complete screening, confirmation, linkage and entry into care within 2 business days.
Because the confirmation of linkage was conducted within chromosomes (see Methods), this lack of linkage can be due either to wrong assignments of SNP sequences to chromosomes or to genotype calling errors.
The reported evidence for "association" might therefore be a confirmation of linkage or mixture of both.
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