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The silica can able to form linkage at the interface of nano CaCO3 and CR matrix.
BG-1 concurrently hydrolyzed α-(1 → 6 -arabinofuranosidic linkage at the C-20 site or outer β-(1 → 2)-glucosidic linkage at the C-3 site of G-Rc to produce G-Rd and C-Mc1, respectively.
C-Mc was also slowly hydrolyzed α-(1 → 6 -arabinofuranosidic linkage at the C-20 site to produce C-K with reaction time prolongation.
C-Mc was also slowly hydrolyzed α-(1 → 6 -arabinofuranosidic linkage at the C-20 site to produce C-K with reaction time prolongation (≥96 -arabinofuranosidic
A significant effect of the orientation and extension of hydrophobic regions around the ether linkage at the 3-position was found on the efficiency of DNA delivery.
In our intein-based and chemical schemes, we employed an intein Mxe or a thioethylbutylamido linkage at the C-terminus of their linear precursors, respectively.
Two candidate tricistronic vector constructs were designed and tested; one with 2A peptide linkage at the N-terminus of hNET (FLuc.2A.RQR8.2A.hNET) and one at the C-terminus (hNET.2A.FLuc.2A.RQR8).
The enzyme also hydrolyzed outer and inner glucosidic linkages at the C-3 site of G-Rd to produce C-K via G-F2, and inner glucosidic linkage at the C-3 site of C-Mc1 to produce C-Mc.
When combined under physiological conditions, the SH32 and kinase domains chemoselectively react through the well-established traditional chemical ligation (Dawson et al., 1994) reaction to form an amide linkage at the ligation junction.
A glucose unit is linked to the C-3 position of the triterpene through ether linkage, and diverse glycosyl groups are linked through an ester linkage at the C-28 position.
Linkage at the opposite side of a flexible hanging arm either via a triple bond or through an amide tether offers the possibility of further use of these ligands for bioconjugation.
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