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The recent Arla acquisition of Milk Link was allowed to proceed.
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Therefore, in the case of distributed antenna configuration selection, when a link is allowed to change its transmit configuration (i.e., TXRA and DSRA), there is the need for an iterative procedure, so as to allow for the other links to respond to the new interference levels caused by transmit configuration changes.
After 2 h, glutaraldehyde solution (200 μL, 25% v/v) was added and cross-linking was allowed to continue for the following 2 h.
Cross-linking was allowed to proceed for 7 min at 37°C and was stopped by the addition of glycine (Sigma) at a final concentration of 125 mM.
Cross-linking was allowed to continue for 10 min at room temperature and stopped by addition of glycine (0,125 M as final concentration), followed by an additional incubation of 5 min. Fixed cells were washed twice with PBS and harvested in SDS buffer (50 mM Tris at pH 8.1, 0,5% SDS, 100 mM NaCl, 5 mM EDTA, and protease inhibitors).
Cross-linking was allowed to proceed for 10 min at 37°C.
Cross-linking was allowed to proceed overnight at 4 °C in a humid atmosphere.
Cross linking was allowed to proceed for 30 min of continued incubation at 30°C.
Cross-linking was allowed to proceed for 15 min at room temperature and then stopped by the addition of glycine to a final concentration of 0.125 M.
Cross-linking was allowed to proceed for 30 minutes at room temperature and stopped by addition of glycine to a final concentration of 0.125 M.
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