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Whilst mutations in lamins A/C have been implicated in a wide range of rare genetic diseases [3], our current findings for the first time link expression of these proteins with progression of one of the commonest causes of cancer-related death in the Western world.
Oncomine centralizes expression profiles of over 3762 microarray experiments covering a broad panel of human cancers and normal tissues and can be used to link expression of novel genes with different grades of tumors, tumor sub-types and, as has been recently shown for CD200 [94].
The ability to specifically link expression levels to multiple cellular responses will be an important means to examine the functional significance of altered protein expression levels sometimes observed in diseases where AR is moderately over-expressed, such advanced metastatic prostate cancer [49].
Using the UniGene IDs it was possible to link expression ratios for 148 genes across the three technologies.
Waters says the rat genome information provides the genetic scaffold for scientists to link expression profiles to a genome that is relevant to toxicology.
An integrated MySQL database was set up and gene identifiers from the UniGene gene index were used to link expression data from the three datasets.
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We then wanted to determine if promoter homology was associated with the linked expression of the two proto-oncogene clusters.
This variable but linked expression was also observed in four different breast carcinoma cell lines tested (Fig. 3B).
Interestingly, we detected linked expression of NKp30L and NKp44L on many cell lines derived from solid tumors.
This is unlikely to be an artifact or a cross-reactivity of our staining reagents as we did not find such linked expression on cells of hematopoietic origin.
IL-8 is also mitogenic [13], angiogenic [14] and, through linked expression of MMP's, influences tumour cell motility such that invasion is enhanced [15].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com