A stabilised cell line of kidney cells (HK2), derived from adult human kidney proximal tubular cells, was used in our experiments (European Cell Culture Collection, Salisbury, UK).
Nevertheless, animal studies and in vitro studies with lines of kidney cells have provided the basis for the conclusion that contrast media can cause kidney injury.
In vitro, several lines of kidney cells have been used to demonstrate that exposure to high glucose results in decreased production of HS GAG [ 52].
Last year, Duanqing Pei of the Chinese Academy of Sciences and his colleagues reported that human urine contains skin-like cells from the lining of the kidney tubules which can be efficiently reprogrammed, via the pluripotent state, into neurons, glia, liver cells and heart muscle cells.
Twelve of the 13 human genome-wide screens have cell viability data available, and this includes two screens of lung-cancer PC9 cell lines, three screens of kidney cancer lines (RCC4, A498 and 786-O lines) and two screens of breast cell lines (MCF-10A and MCF-7).
It's not a pleasant thought, but the bacteria that cause urinary tract infections use tough, hairlike fibers tipped with adhesive to latch onto the lining of our kidneys and bladder.
Monolayers of epithelial cells of the established MDCK line of canine kidney origin (Madin Darby Canine Kidney) were grown on 25 cm cell culture flasks (Corning Incorporated, NY) in Dulbecco's modified Eagle's medium (Microlab, Mexico).
Gaush, C. R., Hard, W. L. & Smith, T. F. Characterization of an established line of canine kidney cells (MDCK).
A low magnification image of a coronal section through the mid-line of the kidney at the level of the renal pelvis is shown in Figure S1.
Paraffin-embedded 5-µm coronal sections through the mid-line of the kidney at the level of the renal pelvis were washed in xylene twice for 5 min each, rehydrated by successive rinsing in 100, 95, 75, and 50% ethanol followed by phosphate-buffered saline (PBS), and antigen retrieval was performed using 10 mmol/l sodium citrate (pH 6.0) with 0.1% Triton X for 20 min at 95°C.
