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Synovial macrophages are positioned throughout the sub-lining layer and lining layer at the cartilage-pannus junction and they mediate articular destruction.
Slides were analysed using a well-established semi-quantitative scoring method (0 4) and scored separately for perivascular/vascular, lining layer and sub-lining layer regions [ 21].
Lining layer thickness was measured by drawing perpendicular lines from the outer surface to the inner margin of the lining layer at intervals of 20 30 μm.
The distribution of ASCs in the lining layer of synovial tissue could also be due to the release of chemokines by macrophages located within the lining layer [ 44].
The lining layer thickness was analyzed by averaging the number of cells in a lining layer cross section at nine different locations (400×).
Meanwhile, the relationship between the MSCs and the FLSs in the lining layer remains unclear.
Activation of JNK in the lining layer of rheumatois arthritis synovium has not been reported.
Some additional staining was present in the lining layer of early RA and PsA samples.
However, the lining layer was CD90-negative (in contrast to podoplanin).
Electron microscopy revealed extensive cell-to-cell contacts within the lining layer [ 1].
Particularly, cells in the lining layer accumulated ApoB within their cytoplasm.
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