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Moreover, cytotoxicity effects of HEPdL in IGR-39 cancer cell lines were tested by MTT assay.
The in vitro antiproliferative activities of the derivatives against A549 and NIH3T3 cell lines were tested by MTT assays.
The structures were confirmed by 1H NMR and MS. Their antiproliferative activities against two cancer cell lines were tested by the MTT method in vitro.
To test whether this was also true at the mRNA level, ten NB cell lines were tested by real-time quantitative PCR (Q-PCR).
The transfection efficiencies of all the cell lines were tested by transfection of the pEGFP-IRESpuro control vector (Clontech, Palo Alto, CA, USA).
A total of 15 primary USC cell lines were tested by RT PCR for the expression of CD46, CD55 and CD59 mCRP at mRNA level.
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Their antiproliferative activity against Bel-7402, HT-1080 and WI-38 cell lines was tested by MTT assay in vitro.
Pluripotency of both lines was tested by embryoid body differentiation, where they gave rise to cell types representative of the three embryonic germ layers (Fig. S1B, C and S2B, C).
Tumorigenicity of the cell lines was tested by injecting 2 × 10 cells subcutaneously into athymic mice.
The authenticity of the cell lines was tested by the DSMZ (German collection of microorganisms and cell cultures).
The recombinant plasmids were co-transfected into COS-7 cells and recognition of the expressed products by Tp1-specific CD8+ T-cell lines was tested by measuring IFN-γ release using an ELISpot assay as described previously 26.
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