Stable cell lines were selected for co-transduction of a puromycin resistance gene and pools of selected cells were used for further analysis.
These two lines were selected for sampling mask.
We ultimately obtained 351 CSSLs, and 127 lines were selected for resequencing.
One and three introgression lines were selected for phenotypic analysis from 9311 and ZH11 genetic backgrounds, respectively.
Therefore, two different GCCLs (N87 and 7901), and two patient-derived cell lines, were selected for further study.
Two hairy root lines were selected for PCR confirmation, whereas A. rhizogenes served as positive control and DNA from non-transformed seedling roots served as negative control.
Based on the preliminary results of PCR and RT-PCR analyses, representative T0 transgenic lines were selected for gene integration analysis.
Among the T1 plants analyzed, two homozygous, 2 heterozygous and 2 null lines were selected for growth analysis in the greenhouse.
All lines were selected for expression of gata3 protein.
Therefore, these two lines were selected for further study.
Homozygous T2 plant lines were selected for further experiments.
