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T-cell lines were raised and expanded as described [38].
Most of the fly lines were raised at 25°C using standard food medium.
The 7 independent F1 lines were raised and outcrossed to create an F2 generation.
AQP422 36 and AQP4289 303 specific T cells were present in the natural T cell repertoire of wild type C57BL/6 mice and T cell lines were raised.
The zebrafish lines were raised with the help of M. Winandy (Zebrafish Facility and Transgenics Platform, GIGA, University of Liège).
Lines were raised on four dietary treatments used in the analysis following a rationale described in detail in Reed et al. (2010).
Similar(54)
Fish from the integrated and segregated hatchery lines are raised in the same facility but are differentially marked for external identification.
Since these cell lines are raised from sporadic cancers, these data provide strong support for a role for BRCA1 in sporadic breast cancer.
From G1 to G8, birds of each line were raised in two hatches.
Mice except GDNF lacZ line were raised in a barrier facility in Hill Pavilion, the University of Pennsylvania.
Zebrafish of the AB wild-type line were raised and staged as described previously [ 32].
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