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Transient transfectants of cell lines were prepared using individual or SMARTpool preparations of small interfering RNA (siRNA) (Thermo Fisher Scientific), targeting the genes of interest.
The metaphase preparations from the cancer cell lines were prepared using standard techniques.
Antibody-capsid conjugates targeting extracellular receptors on human breast cancer cell lines were prepared and characterized.
Encephalitogenic MBP-specific T cell lines were prepared from popliteal lymph nodes of rats with EAE.
All cell lines were prepared for implantation by resuspending cells in DMEM/F12 and Matrigel at a 1 1 ratio.
Various breast cancer cell lines were prepared as paraffin block slides, which were covered by a microfluidic PDMS platform to form a micro-reaction chamber.
HEK cell lines were prepared as described previously [54].
For validation studies, calibration lines were prepared on three successive days, and the average absorbance readings were used to describe the linear regression line for the assay of nifedipine by the CDNBD method.
When the cells grown on 75 cm2 tissue culture flasks reached confluence (usually 24 h), the cell suspension of the three tumor cell lines were prepared in complete growth medium (DMEM).
When the cells reached confluence (usually 24 h), the cell suspension of the three tumor cell lines were prepared in complete growth medium (DMEM) supplemented with 50 µg/ml gentamycin [33].
De novo lymphoclastiod cell lines were prepared.
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