To further validate the interaction between LOX-1 and NadA, binding assays on eukaryotic cell lines were performed.
Cell cycle studies with the complexes in these cell lines were performed to investigate their antitumoral activity.
Tests with positive and negative standard lightning impulse voltages (1.2/50 μs) applied to typical single- and three-phase structures of compact distribution lines were performed.
In addition, fast sequential determination of multiple elements and truly simultaneous determination of Cd, Fe and Ni using nearby analytical lines were performed with the GFA controlled by a single temperature program and an atomization temperature of 2700 °C.
All qRT-PCR experiments for cell lines were performed in triplicate and included no-template controls.
All quantitative real-time polymerase chain reaction (qRT-PCR) experiments in cell lines were performed in triplicate, a minimum of three times and included no-template controls.
Trial management, population generation and phenotypic data collection of progenies comprising of 160 lines were performed using the protocol outlined under lowland conditions.
Biological experiments on Jurkat and MCF-7 cell lines were performed to determine the affinity of our new imaging agents toward the CXCR4 receptor.
In vitro experiment of BSA-NPs cross-linked with GA or denatured by heat against L929 cell lines were performed by CCK-8 to evaluate the cytocompatibility.
Similar tests as those performed with the pGFP-Zenv transgenic lines were performed.
Total RNA extraction of tissues and cell lines were performed with the RNeasy Mini Kit (Qiagen).
