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The absorption spectral lines were measured using an ultraviolet spectrophotometer UV-80000 Shanghaiai Precision instruments Co. Ltd).
The linear distances between the tips of the lips and the five reference lines were measured using digital vernier caliper.
Next, endogenous NA and DMA concentrations in T2 brown rice seeds of 35S, AN1, AN3, and NT lines were measured using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS).
Secretion of activin A and follistatin in conditioned medium (CM) from MDA-MB-231, MDA-MB-436, MCF7 and T47D cell lines were measured using specific ELISAs.
Gene expression levels from NGP and IMR-32 cell lines were measured using Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA).
Soluble proteins in the culture medium of each established stable cell lines were measured using the Human Cytokine Array (RayBiotech, AAH-BLM-1-4, Norcross, GA, USA), according to the recommended protocols.
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Proliferation of these cell lines was measured using BrdU incorporation, and ISO-HAS-B proliferation was elevated compared to primary cultured ECs, whereas that of AS-M was reduced (Supplementary Fig. 8a).
The distribution of HER2 receptor expression on the cell lines was measured using fluorescence-activated cell sorting.
After laser machining of HDPE/MWCNT samples, electrical resistivity of etched lines was measured using a two-probe multi-meter (HIOKI 3280-10).
The migration potential of the cell lines was measured using an established scratch migration assay.
Cytotoxicity of tested sample against different cancer cell lines was measured using the MTT Cell Viability Assay.
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