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Putative transgenic N. benthamiana plant lines were identified by PCR, Southern blot hybridization and RT-PCR.
TAD boundaries (dotted black lines) were identified by an automated algorithm based on computation of the insulation score (plotted below each graph) and application of a threshold.
All constructs were sequenced, and after plant transformation by floral dip70, single copy transgenic lines were identified by Southern blot analyses and representative lines were used for crosses and further analyses.
In order to know whether the mutant phenotype can be rescued, 23 independent transgenic lines were identified by observing the phenotype of leaves at early tillering stage.
To improve the seed viability of susceptible rice genotype Tellahamsa, transgenic plants expressing AKR1 were developed and stable lines were identified by screening on glyphosate as described in our previous study (Vemanna et al., 2016).
Genes with significant difference in expression between cell lines were identified by one-way analysis of variance (ANOVA).
Similar(20)
Cre expression in the two Cre lines was identified by interbreeding with mT/mG mice, which express tomato/EGFP in all tissues.
A comparable number of differentially expressed genes between the pure samples of OCI-Ly8 and HEK293 cell lines was identified by the two technologies.
The cellular expression of Gb3 of the cell lines was identified by a monoclonal rat IgM antibody (Immunotech, Marseille, France) and MDR1/PgP by a monoclonal antibody from Chemicon Internat.
Meanwhile, 653 differentially expressed genes between OS cell lines and an osteoblastic cell line were identified by microarray analysis.
The 1-st report was from Li et al. (Li et al, 2003): 876 myc BSs associated with promoters in the Daudi Burkitt's lymphoma cell line were identified by ChIP-Seq method.
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