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After cells reached 90% confluence, cross lines were generated using a sterile pipette tip.
Stable cells lines were generated using puromycin selection, Dox 1 2 µg/ml, and sorted by red fluorescent protein expression.
Expressing lines were generated using standard mating schemes as described previously [21].
Peptide-specific CD8+ T cell lines were generated using previously described methods [62], [63].
Stable cell lines were generated using blasticidin selection as previously published [24].
Certain lines were generated using facilities of the Duke Model Systems Genomics Group.
Similar(18)
Transgenic lines are generated using germline transformation protocol (Jin, 1999) and previously established selection strategy (He et al., 2010).
All mouse lines are generated using eight genetically diverse founders via a common breeding scheme designed to randomize the genomic contribution of each founder.
Scatterplots and nonparametric regression lines are generated using lowess smoothers [ 13, 14] to illustrate the relationship between radiation dose and clinical endpoints.
The VT collection of molecularly defined enhancer GAL4 lines was generated using the strategy of [ 41] (C. Masser, S.S. Bidaye, A. Stark, and B.J.D., unpublished data).
The Nedp1 −/− and Ubc12 −/− cell line were generated using the CRISPR/CAS9 method.
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