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Two independent transgenic lines were generated and backcrossed for >10 generations into a C57Bl6 background.
Furthermore, stable CHO cell lines were generated and analyzed for antibody production levels and stability.
The Sec61β shRNA stable cell lines were generated following the pLKO.1 protocol (Addgene).
Over 20 independent transgenic lines were generated and used for functional characterization.
All wild-type and mutant transgenic lines were generated in the Oryza sativa L. ssp.
The lines were generated by a stroll Voigt took through a Munich botanical garden.
Two Mad RNAi Drosophila lines were generated.
Lines were generated from the PBMC of healthy donors.
Multiple transgenic lines were generated and tested for each construct.
UASp transgenic lines were generated as described below.
All cell lines were generated at CSMC using standard methods.
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