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The anti-proliferative activities of all synthesized compounds against human hepatocellular (HepG2), cervical (HeLa), and gastric (MGC-803) cancer cell lines were evaluated using an MTT assay.
Their antiproliferative activities against human myelogenous leukemia (K562), human neuroblastoma (SH-SY5Y) and gastric cancer (AGS) cell lines were evaluated using the MTT assay.
The inhibitory activities of the title compounds against the MGC-803 (gastric cancer cell) and Bcap-37 (breast cancer cell) human cancer cell lines were evaluated using standard MTT assay in vitro.
Transcriptional differences between cell lines were evaluated using analysis of variance (ANOVA).
Expressions of t-ERKs and p-ERKs in the two RCC lines were evaluated using Western blotting.
Expressions of cleaved caspase-3 in the two RCC lines were evaluated using Western blotting (Fig. 7A and B).
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The cytotoxicity in vitro of the complex against cancer cells B16, A549, Eca-109, SGC-7901, BEL-7402 anormalmal NIH 3T3 cell lines was evaluated using MTT method.
The line formation like bulged, uniform, and dashed lines was evaluated using an optical microscope, and the variation in line widths of uniform lines was investigated with the process variables.
The cytotoxic activity in vitro of the ligand and complexes toward BEL-7402, A549, HeLa, HepG2 and MG-63 cell lines was evaluated using MTT method (MTT = (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide).
Fold change in expression between sensitive and resistant cell lines was evaluated using the Mann-Whitney test.
HER2 expression of 6 BC cell lines was evaluated using CellSearch® and HER2 Intensity CellSearch® for each cell was calculated as described in the methods.
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