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Their inhibitory activities against the NCI-H460 (lung), A549 (lung adenocarcinoma), HepG2 (liver) and SKOV3 (ovarian) human cancer cell lines were estimated using MTT assay in vitro.
The inhibitory effects of ABL and ABL-N on various carcinoma cell lines were estimated using the MTT cellular survival assay.
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The population structure of 375 lines was estimated using STRCUTURE on 53 well amplified SSR markers evenly distributed on 12 chromosomes (Additional file 1: Table S2).
Wet snow accumulations on overhead power lines are estimated using observations from a network of surface synoptic weather stations, while areas of severe in-cloud icing (rime icing) are identified from high resolution numerical weather prediction model simulations.
Description of cell lines and methods for Western blot can be found in Additional file 3. The cytotoxic effect of methotrexate treatment of PBMCs and cell lines was estimated using a cytotoxicity detection kit (LDH; Roche, Mannheim, Germany).
Linear fits were found for each CLT and confidence intervals for the slope of each line were estimated using 5000 bootstrap repetitions with the BCa method [32].
Size-dependent losses for the TDMPS inlet line were estimated using empirical particle loss corrections (diffusion and gravitation) from Willeke and Baron (1993).
Breeding values of selection candidates in the pedigree sub-line were estimated using the pedigree-based multi-trait BLUP that is used for routine evaluation in the Hy-Line International breeding programs.
The cell volume of each NCI60-panel cell line was estimated using cell size measurements obtained from Cellometer Auto T4 (Nexcelom Bioscience LLC, Lawrence, MA, USA).
The 50% inhibitory concentration (IC50) for 36 hr of treatment with each drug in each cell line was estimated using nonlinear regression (SAS Statistical Software, version 8; SAS Institute Inc., Cary, NC) as described previously (Troester et al. 2004).
Subsequently, line means were estimated using the [([P]ext × Line)] term as a fixed factor, retaining [(Replicate/Run/Plate/Position)] as a random factor.
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