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Both vectors were separately transformed in 29-13 cells, immediately cloned and all phleomycin-resistant cell lines were characterised by immunofluorescence with the anti-PFR2 antibody L8C4.
The carcinoma cell lines were characterised by the two additional H- and A-FABP mRNAs.
These cell lines were characterised by the EuroBoNeT consortium (Ottaviano et al, 2010).
Cell lines were characterised by isoenzyme/cytochrome c oxidase I assay and short tandem repeat analysis by the cell bank.
Importantly, post-EMT and basal cell lines were characterised by IL-8 production whereas it was absent in the luminal-type MCF7 cell line.
All three investigated IRR cell lines were characterised by either slight or either significantly or markedly increased nuclear Rac1 expression compared with parental cells.
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These cell lines are characterised by a population doubling time of more than 150 h and a barely detectable telomerase activity (Table 1).
Fibres from the transgenic B14 line were characterised by a rise in band intensity in the 3000 to 3600 cm-1 range, resulting from different conformations of the intra- and inter-molecular hydrogen bonds in the cellulose.
This KIT exon 11 mutant cell line is characterised by a heterozygous deletion of 57 bases (Taguchi et al, 2002).
The epithelial mesenchymal phenotype for each cell line was characterised by morphological examination and immunofluorescence (IF) staining for prototypic EMT markers.
This cell line is characterised by a KIT exon 13 missense mutation, resulting in a single amino-acid K642E substitution in the proximal part of the cytoplasmic split tyrosine kinase domain (Tuveson et al, 2001).
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Justyna Jupowicz-Kozak
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