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METHODS: Levels of Sp1, Sp3, Sp4, and VEGF proteins in pancreatic cancer cell lines were assessed by immunoblot analysis.

Correlations between %HMGCR13 with %LDLR12 and %LDLR4 among statin or 25-hydroxycholesterol treated immortalized lymphoblast cell lines were assessed by linear regression.

The levels of antibodies induced after immunization with three injections of IrrSpz from the three parasite lines were assessed by ELISA using peptides corresponding to the three domains of CS (N-terminus, repeat region and C-terminus), and by IFA using whole sporozoites.

The gene expression levels of NMDAR2B in NSCLC cell lines were assessed by RT-PCR.

The IC50 values of DM4 and AVE9633 in those cell lines were assessed by MTT assays.

Growth-inhibitory effects of enzastaurin on lung cancer cell lines were assessed by MTS assay.

Finally, the barrier capacity of the four different cell lines was assessed by evaluation of apical-to-basal transport of 50 nm and 490 nm polystyrene beads.

Proliferation of cell lines was assessed by dimethylthiazoldiphenyltetrazoliumbromide (MTT) and 5-bromo-2′-deoxyuridine (BrdU) incorporation assays.

Cold tolerance related to qLTB3 in these lines was assessed by seed fertility.

Protein expression in these cell lines was assessed by Western blot using anti-CHIP rabbit polyclonal antibodies (Calbiochem).

The presence of the T-DNA insertions and homozygosity of insertion lines was assessed by two PCR reactions using the GoTaq master mix (Promega).