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The specificities of the NFL-IRES-CreER and ChAT-IRES-CreER lines were analyzed in flatmounts of adult retina.
In addition, an ovarian cancer transgenic mouse model and 7 murine ovarian cancer cell lines were analyzed in our study.
Both wild-type (WT) lines were analyzed in most experiments and did not show statistically significant differences.
Since only limited numbers of cell lines were analyzed in the present study, this conclusion need further validate in other models.
For quantitative real-time PCR, cDNA samples from human cell lines were analyzed in triplicate with the SYBR® Green Supermix (BioRad) using iCycler Real-Time PCR Detection System (BioRad).
18 human PDAC cell lines were analyzed in vitro.
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As shown in Figure 3D, the DNA hypermethylation status of 7 genes showing dense CpG island methylation in breast cancer cell lines was analyzed in 24 primary breast cancer and paired normal breast samples.
Additionally, the role of KPNA2 in the proliferation, migration and apoptosis of urothelial carcinoma cell lines was analyzed in vitro.
The observed suppression of patatins in patatin hpRNAi lines was analyzed in more detail using two-dimensional (2D) gel electrophoresis.
The rat glioma cell line A15A5 was stably transfected with human Bcl-2 and Bax transgenes and the viability of theses cell lines was analyzed in vitro and in vivo.
For analysis of the non-fixed cells, 0.1 μg of protein lysate from each of the 4 cell lines was analyzed in triplicate in the EGFR ELISA immunoassay and quantitative EGFR determined for each lysate by comparison to the standard curve.
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