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Transgenic and non-transgenic cell lines were analyzed for cell viability using a Vi-Cell XR Cell Viability Analyzer (Beckman Coulter, Brea, CA, USA).
(c) The established cell lines were analyzed for live cell imaging for fluorescent signals.
Transformed lines were analyzed for p24 protein content by western blotting using anti-p24-specific antibodies and by Southern blotting to establish the number of copies of the insert in the plant nuclear genome.
METHODS: Five metastatic prostate cancer cell lines were analyzed for p16 gene structure and its expression by Southern and Northern blot analyses.
In a second set of experiments, TH cell lines were analyzed for T cell receptor Vβ chain (TCR-Vβ) expression.
Several human kidney cell lines were analyzed for cadherin-9 expression on the mRNA and protein levels.
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The differential expression in sensitive and resistant lung cancer cell lines was analyzed for each signature gene.
As a pre-requisite to functional studies, a panel of human cancer cell lines was analyzed for MTUS1 expression by real-time RT-PCR (Supplemental Fig. S2A).
The fruit of the citrange lines was analyzed for an additional season (2007; S4).
Finally, a panel of 23 lung cancer cell lines was analyzed for the presence of introns retained in the mature mRNA (Additional file 2: Table S4).
In addition, four tumors from each cell line were analyzed for the presence of senescent cells by SABG staining, as described previously [10].
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