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Antibodies: PD1 surface expression in mouse peripheral blood lymphocytes (PBL) and mouse cell lines was analyzed using PT85 anti-SLA antibody (VMRD Inc, Pullman, WA) while human cell lines were screened using the anti-SLA antibody 74-11-10 (VMRD).
In the present study, the genetic diversity of seven genotypes resistant to shoot fly and/or stem borers, a susceptible control genotype and two susceptible elite hybrid parental lines was analyzed using PCR amplification product differences revealed by 26 ISSR primers.
To estimate allergenic potential of the rice seeds of these lines, IgE binding of seed protein extracts from two Major x HMW lines was analyzed using immuno-dot blotting using serum specimens from 10 allergic patients with relatively high levels of serum IgE to rice seed proteins.
Expression of PGL34 g - YFP in these lines was analyzed using both Western blotting and fluorescence microscopy.
The correlation between the sialyltransferase gene expression and activity, in the cell lines, was analyzed using Spearman and Pearson methods.
Telomerase activity in tumor cell lines was analyzed using the TRAPeze Telomerase Detection Kit (Chemicon International a division of Serologicals Co., Temecula, CA).
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Assumptions needed for the simplified treatment of the 3D small-scale governing equation on lines are analyzed using DNS data of well-developed turbulent channel flow.
Microbial populations inhabiting the bulk soil, and those colonizing the root system of the two tobacco lines, were analyzed using cultivation-independent (phospholipid fatty acid and denaturing gradient gel electrophoresis) and cultivation-based assays.
Expression patterns of CHIT2, PR10, and Betv1 in OsWRKY11-kd lines were analyzed using RT-PCR with gene-specific primers.
In order to isolate the role of OsINV3 in spikelet cell expansion, spikelets at 2 days before heading (DBH) from WT, mutant and the C4 lines were analyzed using scanning electron microscopy (SEM).
The levels of secreted SFRP3 from the different cell lines were analyzed using a commercial sandwich ELISA kit (R&D Research Minneapolis, MN).
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