Antibodies: PD1 surface expression in mouse peripheral blood lymphocytes (PBL) and mouse cell lines was analyzed using PT85 anti-SLA antibody (VMRD Inc, Pullman, WA) while human cell lines were screened using the anti-SLA antibody 74-11-10 (VMRD).
In the summer of 2011, the seed setting rate of 52 testcross F1 lines was analyzed.
a The expression of OsWRKY80 in different overexpression lines was analyzed by RNA gel blot.
The proliferative response to somatostatin in pancreatic cancer cell lines was analyzed by incorporation of [3H]-thymidine.
To evaluate the ability of insect cells to produce complex-type N-glycans, β-(1→4 -galactosyltransferase (β4GalT) activity in several insect cell lines was analyzed.
The uptake of the nanoparticles by the different cell lines was analyzed through granularity (side scatter of light (SSC-H) vs. forward scatter of light (FSC-H)) changes on FACSCalibur flow cytometer (Becton Dickinson, USA).
Available Pi content of positive T1 seeds of individual transgenic lines was analyzed (Kuwano et al. 2009; Chen et al. 1956) and compared with the levels of non-transgenic rice seeds.
After the transformation, more than 10 transgenic lines displayed early flowering and stunted growth, and this early flowering phenotype associated with OsMADS45 expression observed in the progenies of various transgenic lines was analyzed and verified for more than three generations.
To estimate allergenic potential of the rice seeds of these lines, IgE binding of seed protein extracts from two Major x HMW lines was analyzed using immuno-dot blotting using serum specimens from 10 allergic patients with relatively high levels of serum IgE to rice seed proteins.
In the present study, the genetic diversity of seven genotypes resistant to shoot fly and/or stem borers, a susceptible control genotype and two susceptible elite hybrid parental lines was analyzed using PCR amplification product differences revealed by 26 ISSR primers.
The carotenoid composition of tubers and leaves from selected lines was analyzed via diode array HPLC.
