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The relative expression of BTG2, CNN3, ID3, RGS1 and F13A1 in ICF patient and control cell lines was analysed using the Bio-Rad iCycler system.
Expression of UGT8 in breast cancer tissue specimens and breast cancer cell lines was analysed using IHC, real-time PCR and Western blotting.
Fertilization and meiosis in all lines was analysed using standard in vitro fertilization and ookinete maturation assays (van Dijk et al., 2010).
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Genomic DNA samples from the eight medulloblastoma cell lines were analysed using the GeneChip® Mapping 10K 2.0 Array (Affymetrix), and raw data processed using Microsoft Excel spreadsheets, as previously described [13].
The gene expression changes in DoxHPC1 and DoxHPC7 cell lines were analysed using the hybridization scheme in Figure 2A.
Rho activity in wild type, heterozygous and homozygous cell lines were analysed using the active Rho-GTP pull down assay.
Data from proliferation curves between Li-treated and untreated cell lines were analysed using non-parametric Wilcoxon rank sum tests.
Changes in the gene expression and methylation levels in ESCC cell lines were analysed using gene expression microarrays and the Infinium HumanMethylation450K BeadChip assay, respectively.
The normalised data from the various cell lines were analysed using the Bio-Rad CFX manager software 3.0 and fold change determined.
The microarray data from the four normal colon cell lines were analysed using the Anduril bioinformatics framework (Ovaska et al, 2010).
To produce a comprehensive survey of genomic aberrations in BC, five BC cell lines were analysed using array-CGH for patterns of chromosomal gains and/or losses.
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