The father Abel's opening lines are adapted from Emerson's writings about the death of his firstborn.
These marionettes, however, take the form of mechanized insects, and their recorded lines are adapted from García Lorca's play about a family vendetta, "Blood Wedding".
The straight lines are adapted from references [22].
SBCC winter lines are adapted to a typical mild Mediterranean winter, in which temperatures are not very low.
Parental chemosensitive cell lines were adapted to growth in the presence of anti-cancer drugs by continuous exposure of these cell lines to the increasing concentrations of these drugs as described before.
At passage 2 3, iPSC lines were adapted to grow on Matrigel (human ES-qualified Matrix from BD Biosciences, Franklin Lakes, NJ, USA) in mTESR1 medium (Stem Cell Technologies, Vancouver, BC, Canada) as described.
All BE cell lines were adapted to serum-free conditions in keratinocyte serum-free (KSF) medium supplemented with bovine pituitary extract and epidermal growth factor (EGF) (Invitrogen Corp. Carlsbad, CA) by serial passaging of cell lines into successively lower concentrations of serum until the cell lines survived without serum present.
All cell lines were adapted for growth in Dulbecco's modified Eagle's medium (DMEM, Cambrex-Biowhittaker Walkersville, MD) supplemented with 0.1 Unit/ml bovine insulin (Sigma Chemical Co. St . Louis MO), 100 I. U. penicillin, 100 μg/ml streptomyocin, 0.25 μg/ml amphotericin B (Cellgro Herndon, VA) and 10% fetal bovine serum (FBS Hyclone, Logan UT).
This cell line was adapted to grow in suspension and in serum-free medium.
The Bury line was closed in stages between 13 July 1991 and 17 August 1991, after which the 1200V DC third rail electrified line was adapted for a 750V DC overhead line operation.
