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Quantitation of SU101 and SU0020 in these cell lines was achieved by a specific and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method.
Although the maximal effect for different cell lines was achieved at different concentrations of glucosamine and at different treatment time (not shown), for 8 of 9 cell lines a statistically significant increase in the activity was observed (Figure 5C), suggesting that some of the mutants were partially stabilized by the glucosamine, correctly processed and targeted to the lysosomal compartment.
Generation of transgenic lines was achieved as described previously (Kawasaki et al. 2004).
A partial reduction of the PAM copies in the CHO Der2 and CHO Der3 cell lines was achieved.
Disease control (PR or SD) by any of the sequential therapy lines was achieved in 15 patients (42.8%).
The HSF1 over-expression in these two cell lines was achieved using lentiviral tetracycline inducible expression system (TetR-HSF1).
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Linear calibration lines were achieved for experimentally and theoretically determined differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRPD) data.
Through proper shielding design, exceptionally low crosstalk between adjacent electrode lines is achieved, which gives an optimal near end crosstalk (NEXT) value of 0.01.
As in so much dance modernism (whether modern dance or ballet), these lines were achieved with terrific, dynamic excitement: you didn't just admire a shape, you felt the thrust involved in making it.
It was reported that gratings of 70-nm lines were achieved using UV R2R NIL, with an imprint speed up to approximately 1,400 mm/min.
Knockdown experiments in MPM cell lines were achieved by transiently transfecting cells with the indicated specific small interference RNAs (siRNAs) or control siRNA (csi), purchased from Sigma-Aldrich.
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CEO of Professional Science Editing for Scientists @ prosciediting.com