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These antibodies showed specific binding to synthetic mutant peptide in Biacore, ELISA and Western blot assays, but did not detect a mutant BAX protein band in whole cell lysates or immunoprecipitates of BAX mutated cell lines (unpublished data).
These lines were selected as extremes with respect to DNDF from a larger set of > 300 European lines (unpublished data).
Indeed, we found that the Wnt ligand Wnt-3a repressed glucose production in primary hepatocytes and cultured hepatic cell lines (unpublished data of Ip and Jin).
And, we also found elsewhere an increase in pS2 mRNA in MCF7, HBL100 and MDA-MB-231 human breast cancer cell lines (unpublished data).
Considering the effect of genistein on growth inhibition in RCC cell lines (unpublished data), it may be a novel therapeutic agent for RCC patients.
The result was in agreement with GalNAc-T14 expression revealed by semi-quantitative RT-PCR in the cells lines (unpublished data).
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For Puf2 we also generated a second independent knockout clone, which was phenotypically identical to the first puf2 clonal parasite line (unpublished data).
Two Taiwanese clinical isolates of HP238 (from gastric adenocarcinoma specimen) and HP250 (from mucosa-associated lymphoid-tissue lymphoma speciment) can multiply in macrophage cell line THP-1 [8] whereas standard strains of ATCC43504 and J99 can multiply in AGS or MKN45 epithelial cell line (unpublished data).
v2 – E. Blumwald and J. Sottosanto, arabidopsis, A. thaliana ecotype Wassilewskija, wild-type line (WS), and a nhx1 'knockout' line (unpublished data).
The main metabolite (M23) is not active against Plasmodium falciparum and shows no cytotoxic potential against a human hepatic cell line (unpublished data).
The expression levels of some pluripotency genes (Oct4, Sox2, Rex1, and FoxD3) were analyzed for the same rAT-MSC line (unpublished data).
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